Protocols Breeding Schemes
The mutagenized (G0) mice are not expected to show
abnormal phenotypes, but they can be bred with a normal
female to generate G1 offspring that are heterozygous for
mutations induced in the G0 male. In order to create animals that are
homozygous for mutations carried by the G1 males 2 further
generations of breeding are required to produce G3 animals.
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Breeding schemes to detect recessive mutations in
third-generation (G3) offspring. In the backcross scheme
, G1 male progeny are mated with wild-type
females to produce second-generation (G2) progeny. The
female G2 progeny are then bred with their G1 father to
produce G3 progeny. Dominant and semidominant mutations are
expressed in an average of 75% of the G3 offspring.